華中農(nóng)業(yè)大學(xué)

基本信息姓名: 董五輩 出生年月: 1965.2
性別: 男 碩/博導(dǎo)： 博導(dǎo)
民族: 漢 開設(shè)課程： 分子植物病理學(xué)（留學(xué)生和本科生），分子生物學(xué)（研究生）
職稱: 教授 研究方向： 抗病基因克隆；植物-微生物分子互作應(yīng)用工程
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學(xué)位: 農(nóng)學(xué)博士

聯(lián)系方式 辦公電話：
移動電話：15007109436
電子郵件：dwb@mail.hzau.edu.cn

個人簡介董五輩，男，1965年2月出生，博士生導(dǎo)師。主要從事于廣譜抗病基因的分離克隆和植物-微生物分子互作應(yīng)用工程。利用我們實驗室建立的新技術(shù)從中草藥植物及生防菌等中分離抗病基因。利用我們分離到的新基因轉(zhuǎn)化植物及生防菌。我們在《The Plant Cell》，《PNAS》，《Scientific Reports (Nature)》，《Virology》，《PLoS ONE》，《Plant Physiology》，《IET Systems Biology》，《Molecular Plant Pathology》，《農(nóng)業(yè)生物技術(shù)學(xué)報》等國內(nèi)外刊物上發(fā)表文章30多篇。包括《ScienceDaily》，《Economist》，《Daily Science News》，《United Press International》，《Medical News Today》，《NewsZoom》，《The Sunday Paper》，《Bio-Medicine》，《What's Next In Science & Technology》，《UGA NEWS》等至少33家國際媒體曾先后報道了我們的研究工作，如本實驗室建立的基因消除PCR技術(shù)被Nature出版集團發(fā)表在《科學(xué)報道》上后，又被美國的一家科技信息媒體作為新發(fā)明進行了報道，詳情請參見下面網(wǎng)頁：http://www.sciguru.com/newsitem/16371/reverse-process-pcr-eliminate-unwanted-genes-cycle-cycle-new-invention 。

基因消除PCR(Removing PCR, R-PCR)是基因擴增PCR逆向過程的一種去除非目標(biāo)基因的新技術(shù)。在PCR反應(yīng)中，每一循環(huán)目標(biāo)基因得到了擴增；在基因消除PCR反應(yīng)中，每一循環(huán)非目標(biāo)基因得到了去除。如果你想擴增你想要的基因，你可用PCR；如果你想去除你不想要的基因，你可用基因消除PCR。

有關(guān)教學(xué)方面的一些工作請參見網(wǎng)站：http://mpp.hzau.edu.cn/

（一）學(xué)歷
1982—1986: 生物學(xué)本科學(xué)士, 華中師范大學(xué)
1992—1995: 碩士研究生, 遺傳學(xué)專業(yè), 華中師范大學(xué)
1998—2001: 博士研究生, 作物遺傳育種專業(yè), 中國農(nóng)業(yè)大學(xué)
（二）工作經(jīng)歷
2002.01—2002.08 荷蘭瓦赫寧根大學(xué)植物病理學(xué)專業(yè) 博士后
2002.09—2005.02 德國遺傳學(xué)與作物研究所(IPK Institute) 博士后研究員
2005.02—2007.12 美國佐治亞大學(xué)遺傳學(xué)專業(yè) 博士后
2008.01—2009.08美國特拉華州立大學(xué) 助理研究員及兼任講師(Research Associate & Adjunct Instructor).
2009.09—現(xiàn)在 華中農(nóng)業(yè)大學(xué)植物病理學(xué)專業(yè) 教授
（三）學(xué)術(shù)兼職
美國Sigma Xi, the Scientific Research Society學(xué)會會員
湖北省植物病理學(xué)會理事

科研項目2009年華中農(nóng)業(yè)大學(xué)海外人才引進項目 2009 — 2012 100萬元 主持
華中農(nóng)業(yè)大學(xué)自主科技創(chuàng)新基金資助項目 2010.01 — 2011.12 30萬元 主持
抗除草劑、抗紋枯病等轉(zhuǎn)基因育種新材料獲得(課題編號：2011ZX08003-001)，2011 — 2015，轉(zhuǎn)基因生物新品種培育國家科技重大專項，子課題主持人。
大小麥與白粉病菌互作的分子機制研究，中德合作科研項目（PPP），2011 — ， 中方主持人。

教學(xué)研究項目：
校級教學(xué)研究項目：植物病理學(xué)外國留學(xué)研究生英語授課課程建設(shè) 2010 — ， 5萬元，主持
湖北省高等學(xué)校省級教學(xué)研究項目：植物生產(chǎn)類學(xué)科基礎(chǔ)課程植物病理學(xué)全英語教學(xué)模式研究與實踐（項目批準(zhǔn)號：2012178），2012 — ，主持

發(fā)明專利及獲獎情況主持申請的專利：
1. 基因消除PCR（ZL 201110382308.3）
2. 一種文庫對文庫酵母雙雜交規(guī)模化篩選互作蛋白質(zhì)的方法（201210395295.8）

湖北省優(yōu)秀碩士學(xué)位論文指導(dǎo)老師，2014
華中農(nóng)業(yè)大學(xué)2013年大學(xué)生課外學(xué)術(shù)科技作品競賽“優(yōu)秀指導(dǎo)老師”
湖北省品牌課程《分子植物病理學(xué)》, 2013
華中農(nóng)業(yè)大學(xué)教學(xué)優(yōu)秀質(zhì)量二等獎, 2011

發(fā)表的論文及著作發(fā)表的論文：
1. Tingting Zhang, Yinhui Jiang, and Wubei Dong*. 2014. A novel monopartite dsRNA virus isolated from the phytopathogenic fungus Ustilaginoidea virens and ancestrally related to a mitochondria-associated dsRNA in the green alga Bryopsis. Virology 462-463：227–235. DOI: 10.1016/j.virol.2014.06.003.(SCI, 影響因子 3.367).

2. Mary E Case, James Griffith, Wubei Dong, Ira L Tigner, Kimberly Gaines, James C Jiang, S Michal Jazwinski, Jonathan Arnold. 2014. The aging biological clock in Neurospora crassa. Ecology and Evolution 4(17): 3494–3507 (SCI, 影響因子1.658).

3. Jiaojiao Huan, Kangkang Wan, Yunjun Liu, Wubei Dong*, and Guoying Wang. 2013. Removing PCR for the elimination of undesired DNA fragments cycle by cycle. Scientific Reports 3, 2303; DOI:10.1038/srep02303 (2013) (SCI, 影響因子 5.078). http://www.nature.com/srep/2013/130729/srep02303/full/srep02303.html

4．Tingting Zhang, Yinhui Jiang, Junbin Huang, and Wubei Dong*. 2013. Genomic organization of a novel partitivirus from the phytopathogenic fungus Ustilaginoidea virens. Archives of Virology. 158(11): 2415–2419. DOI 10.1007/s00705-013-1742-3 (SCI, 影響因子 2.282).

5．Tingting Zhang, Yinhui Jiang, Junbin Huang, and Wubei Dong*. 2013. Complete genome sequence of a putative novel Victorivirus from Ustilainoidea virens. Archives of Virology 158(6): 1403–1406. DOI: 10.1007/s00705-013-1615-9 (SCI, 影響因子 2.282).

6. R.V. Chowda Reddy, Wubei Dong, Thomas Njock, M.E.C. Rey, and Vincent N. Fondong. 2012. Molecular interaction between two cassava geminiviruses exhibiting cross-protection. Virus Research 163: 169-177 (SCI, 影響因子2.846).

7. Xiaojia Tang, Wubei Dong, James Griffith, Roger Nilsen, Allison Matthes, Kevin B. Cheng, Jaxk Reeves, H.-Bernd Schuttler, Mary E. Case, Jonathan Arnold, and David A. Logan. 2011. Systems Biology of the qa Gene Cluster in Neurospora crassa. PLoS ONE 6(6): e20671. doi:10.1371/journal.pone.0020671 (SCI, 影響因子 3.534).

8. Nina Zellerhoff, Axel Himmelbach1, Wubei Dong, Stephane Bieri, Ulrich Schaffrath, and Patrick Schweizer. 2010. Nonhost resistance of barley to different fungal pathogens is associated with largely distinct, quantitative transcriptional responses. Plant Physiology 152(4): 2053-2066 (SCI, 影響因子 7.394).

9. R.V. Chowda-Reddy, Wubei Dong, Christian Felton, Danielle Ryman, Keith Ballard, and Vincent N. Fondong. 2009. Characterization of the cassava geminivirus transcription activation protein putative nuclear localization signal. Virus Research 145(2): 270–278 (SCI, 影響因子 2.429).

10. Wubei Dong, X. Tang, Y. Yu, R. Nilsen, R. Kim, J. Griffith, J. Arnold, and H-B Schuttler. 2008. Systems Biology of the Clock in Neurospora crassa. PLoS ONE 3(8): e3105. doi:10.1371/journal.pone.0003105 (SCI, 影響因子 3.534).

11. Wubei Dong, X. Tang, Y. Yu, J. Griffith, D. Choi, J. Baldwin, L. Hilton, K. Kelps, J. McGuire, R. Morgan, M. Smith, Q. Wang, J. Liu, J. Reeves, D. Logan, M. Case, and J. Arnold, & H-B Schuttler. 2007. Systems Biology of the Neurospora Biology Clock. IET Systems Biology 1(5): 257-265 (SCI, 影響因子 1.955).

12. Yihai Yu, Wubei Dong, Cara Altimus, Xiaojia Tang, James Griffith, Melissa Morello, Lisa Dudek, Jonathan Arnold, and Heinz-Bernd Schüttler. 2007. A genetic network for the clock of Neurospora crassa. Proc Natl Acad Sci USA. 104(8): 2809-2814 (SCI, 影響因子 9.598).

13. David A. Logan, Allison L. Koch, Wubei Dong, James Griffith, Roger Nilsen, Mary E. Case, Heinz-Bernd Schüttler, and Jonathan Arnold. 2007. Genome-wide expression analysis of genetic networks in Neurospora crassa. Bioinformation 1(10): 390-395.

14. Wubei Dong, Daniela Nowara, and Patrick Schweizer. 2006. Protein Polyubiquitination Plays a Role in Basal Host Resistance of Barley. The Plant Cell. 18:3321-3331 (SCI, 影響因子 9.653).

15. Jonathan Arnold, Wubei Dong, James Griffith, David Choi, Joshua Baldwin, Levi Hilton, Kristen Kelps, James McGuire, Rachel Morgan, Marquita Smith, Qin Wang, Jing Liu, Jaxk Reeves, David Logan, Mary Case and H-Berndt Schuttler. 2006. Systems Biology of the Biological Clock. IEEE Proceedings of the First International Conference on Computational Systems Biology (ICCSB-2006), pp. 119-125.

16. Wubei Dong, Maita Latijnhouwers, Rays H.Y. Jiang, Harold J.G. Meijer, and Francine Govers. 2004. Downstream targets of the Phytophthora infestans G subunit PiGPA1 revealed by cDNA-AFLP. Molecular Plant Pathology. 5(5 ), 483–494 (SCI, 影響因子 4.485).

17. Wubei Dong. 2004. Pathogenicity target gene is the Achilles' heel in host-pathogen interaction. Cereal Rusts and Powdery Mildews Bulletin [www.crpmb.org/] 2004/1029dong [http://www.crpmb.org/2004/1029dong/index.htm].

會議論文摘要：
1. Jiaoiao Huan, Kangkang Wan, and Wubei Dong. A novel removing PCR method for isolation of Blumeria specialized genes. 10th International Congress of Plant Pathology. Beijing, China, August 25-30, 2013.

2. Wubei Dong. ISOLATION OF BARLEY NONHOST RESISTANT COMPONENTS TO BLUMERIA GRAMINIS. 13th International Cereal Rusts and Powdery Mildews Conference. Beijng Friendship Hotel, China, 28 August - 1 September, 2012 (大會報告).

3. Wubei Dong. Pathogen agent genes in plants. The 2nd International Conference on Biotic Plant Interactions. Kunming, China, November 12-16, 2011 (Abstract).

4. Tingting Zhang, Jiaojiao Huan, Jue Huang, Junwei Shi, Menglan Cheng, Wei Kuang, Wubei Dong*. What we can learn from high similarities of molecular mechanisms between barley host and nonhost resistances to Blumeria graminis. PHYTOPATHOLOGY, 2011, 101(6): S202-S202 (Abstract).

5. Dong, Wubei, Xiaojia Tang, Yihai Yu, Roger Nilsen, James Griffith, Jonathan Arnold, and H.-Bernd Schuttler. Systems Biology of the Biological Clock. XXIV FUNGAL GENETICS CONFERENCE Asilomar California，USA. March 20-25, 2007 (http://www.fgsc.net/asil2007/xxivFGCposterAbs.htm).

6. Wubei Dong, Y. Yu, C. Altimus, J. Griffith, M. Morello, L. Dudek, H.-B. Schuttler, & J. Arnold. Systems Biology of the Biological Clock. Neurospora 2006 Conference, Asilomar California, USA. March 30 - April 2, 2006 (http://www.fgsc.net/asil2006/2006PosterAbstracts.htm).

7. Dimitar Douchkov, Wubei Dong, Uwe Zierold, Daniela Nowara, Udo Seiffert and Patrick Schweizer. High throughput RNAi to study the interaction between barley and powdery mildew fungi (http://www.pflanzen-biotechnologie.de/Abstracts/Schweizer.html).

8. Dimitar Douchkov , Wubei Dong , Uwe Zierold , Patrick Schweizer. 2006. High-Throughput RNAi In Barley Reveals Genes Involved In Durable Pathogen Resistance. Plant & Animal Genomes XIV Conference. January 14-18, 2006. Town & Country Convention Center San Diego, California，USA.

9. Wubei Dong, 2004. Pathogenicity target gene is the Achilles’ heel in host-pathogen interaction. Proceedings of the 11th International Cereal Rusts and Powdery Mildews Conference, Norwich, England, 22-27 August 2004, abstract 2.80, Cereal Rusts and Powdery Mildews Bulletin. (http://www.crpmb.org/icrpmc11/abstracts.htm#A2_80)

10. Wubei Dong, Dimitar Douchkov and Patrick Schweizer, 2004. Comparison of gene expression profiles in barley epidermis in response to Blumeria graminis f.sp. hordei and Blumeria graminis f.sp. tritici. Proceedings of the 11th International Cereal Rusts and Powdery Mildews Conference, Norwich, England, 22-27 August 2004, abstract 1.3, Cereal Rusts and Powdery Mildews Bulletin. (http://www.crpmb.org/icrpmc11/abstracts.htm#A1_3)

11. Uwe Zierold, Wubei Dong, and Patrick Schweizer. 2004. Transcriptome analysis in the epidermis of disease-resistant barley. Abstracts Mini Symposium on Expression Data Analysis. (http://pgrc-16.ipk-gatersleben.de/~pdw/exd/Abstracts.pdf)

12. Dimitar Douchkov, Wubei Dong, Daniela Nowara and Patrick Schweizer. Functional analysis of nonhost resistance in barley by RNAi. Plant GEMs Lyon 2004: 3th Plant Genomics European Meetings. 22 - 25 September 2004. Lyon, France

13. Dimitar Douchkov, Wubei Dong and Patrick Schweizer. An and RNAi-based approach to nonhost disease resistance in barley (GABI-NONHOST). Plant GEMs Lyon 2004: 3th Plant Genomics European Meetings. 22 - 25 September 2004. Lyon, France

14. Rays Jiang, Wubei Dong, Maita Latijnhouwers, Jun Guo, and Francine Govers. Transcriptional profiling in Phytophthora infestans using cDNA-AFLP. Phytophthora Molecular Genetics Network Asilomar Conference Grounds, Pacific Grove, California, USA. March 16-18, 2003

15. Maita Latijnhouwers, Wubei Dong and Francine Govers. The Functions of a G-alpha and a G-beta Protein in the Life Cycle of the Oomycete Phytophthora Infestans. Phytophthora Molecular Genetics Network Asilomar Conference Grounds, Pacific Grove, California, USA. March 16-18, 2003

16. Dimitar Douchkov, Wubei Dong, Uwe Zierold and Patrick Schweizer. Development of a high-throughput gene-silencing system for functional analysis of nonhost resistance in barley. RNAi: the technology to revolutionise functional genomic research—What are the limitations? 20 - 22 November 2003. Harnack-Haus, Berlin-Dahlem, Germany.

17. Schweizer, P., D. Douchkov, and W. Dong. Reverse-genetic screening by RNAi for nonhost disease resistance in barley. Presentations during the 3rd GABI status seminar (11./12. February 2003, GSI Bonn, Germany)

18. Schweizer, P., D. Douchkov, & W. Dong: Silencing the transcriptome of disease-resistant barley. 11. International Congress of Molecular Plant-Microbe Interactions. St. Petersburg/Russia, 18-25.07.2003

19. Zierold, Uwe; Douchkov, Dimitar; Dong Wubei; and Schweizer, Patrick. SILENCING THE TRANSCRIPTOME OF DISEASE-RESISTANT BARLEY. The International Joint Workshop on PR-Proteins and Induced Resistance Hotel Marienlyst – Elsinore Denmark, May 5-9, 2004 http://pr-ir2004.risoe.dk

20. Jiang, R.H.Y.; Jun Guo, --; Latijnhouwers, M.; Dong, W.; Govers, F. (2003)
Transcriptional profiling in Phytophthora infestans using cDNA-AFLP
In: Proceedings of The Dutch-Chinese Life Science Forum, Wageningen, The Netherlands, 12 October 2003. - [S.l.] : [s.n.], - p. 28.

21. Latijnhouwers, M.; Dong, W.; Govers, F. (2003)
The functions of a G-alpha and a G-beta protein in the life cycle of the oomycete Phytophthora infestans
In: Book of Abstracts XXII Fungal Genetics Conference, Asilomar, USA, 18-23 March 2003. - Asilomar, USA : [s.n.], - p. 148.

22. Ligterink, W.; Dong, W.; Jiang, R.H.Y.; Guo, J.; Latijnhouwers, M.; Slot, A. van 't; Vondervoort, P. van de; Weide, R.; Govers, F. (2002)
Phytophthora research at Wageningen University
In: Book of Abstracts : Phytophthora Molecular Genetics Workshop, 1-2 August 2002, Milwaukee Wisconsin, USA. - [S.l.] : [s.n.], 2002 - p. 6