西北農林科技大學

1．基本信息

王喬春，男，1958年2月生，四川眉山人，教授，博士生導師。1982年畢業于四川農業大學園藝系，獲學士學位，同年留校任助教。1988年畢業于瑞典農業大學園藝系，獲碩士學位。1988-1995年，在四川農業大學園藝系任助講師、副教授、系副主任、林學園藝學院常務副院長。1994年在奧地利維也納農業大學應用微生物系作高級訪問學者。1995-1998年在四川省農業科學院園藝所任所長、研究員。2003年畢業于以色列西伯萊大學，獲博士學位，同年在該校完成博士后工作。2003-2008年在芬蘭赫爾辛基大學應用生物系作訪問教授。2007年回國，在西北農林科技大學園藝學院任教授，博士生導師。

現任園藝學院副院長，國際Cryobiology Society會員、國際 In Vitro Scoiety 會員、國際園藝學會Cryo-Technology 分會學術委員會委員，應邀為《Trends in Plant Science》、 《Cryobiology》、 《CryoLetters》、 《Plant Cell, Tissue and Organ Culture》、 《Euphytica》、《Scientia Horticulturae》、《Plant Science》、《Plant Cell Reports》、《Potato Research 》等國際SCI刊物特約審稿人。

2．研究方向

主要從事園藝植物種質資源的超低溫保存技術與理論研究，園藝植物超低溫脫毒技術與理論研究，園藝植物再生體系的建立與轉基因技術研究，園藝植物無性繁殖技術與理論研究。

3. 開設課程

承擔本科生、碩士和博士研究生園藝學導論、園藝植物病毒學、SCI論文寫作、科研專題講座、現代生物技術、園藝學專題講座等課程。

4．主要學術成果

A．1984-1990年間，主要研究扦插繁殖機理與技術。以Hibiscus rosa-sinensis為試材, 首次在國際上報道母株年齡和光照條件是影響插條生根的關鍵因素。明確指出，適合于Hibiscus rosa-sinensis扦插繁殖的母株年齡為3年，母株暗處理或遮光處理有利于插條生根。上述研究在國際刊物上發表5篇論文，其中在SCI刊物上發表1篇，應邀參加國際學術會議，并作大會發言。

B．1991-1995年間，主要研究蘋果與梨試管苗繁殖的機理與技術。揭示了蘋果與梨莖尖外植體褐變的機理；建立了克服蘋果與梨莖尖外植體褐變的有效方法、促進蘋果與梨試管苗增殖的關鍵技術、梨試管苗的直接生根技術及蘋果試管苗微型嫁接技術。上述研究在國際刊物上發表11篇論文，其中在SCI刊物上發表論文6篇，應邀參加國際學術會議兩次，并作大會發言。

C．1999-2008年間，主要研究園藝植物與薯類植物的超低溫冷凍機理與技術，轉基因技術和分子生物學技術。對國際該領域的主要學術貢獻包括：a. 成功地建立了葡萄、柑桔、馬鈴薯、甘薯、樹梅等植物的莖尖和細胞的超低溫冷凍保存技術；b. 把超低溫冷凍技術與轉基因技術結合，顯著提高葡萄轉基因細胞的轉化率與成苗率；c. 成功地建立了葡萄、馬鈴薯、甘薯等植物的莖尖超低溫冷凍脫毒技術和脫除甘薯植原體技術；d. 成功地創立了熱處理加莖尖超低溫冷凍脫毒技術，有效地脫除了樹莓叢狀矮化病毒; e. 利用生物技術、分子生物學技術、組培技術、超低溫冷凍技術、病毒定位技術、電鏡技術、解剖技術等揭示了莖尖超低溫冷凍脫除病原菌的機理。成為國際上莖尖超低溫冷凍脫除病原菌研究的奠基人之一。上述研究在SCI刊物上發表18篇論文，發表專著章節5章。應邀參加國際學術會議三次，并作大會發言。
 
5．在國際刊物上發表的主要論文與著作

Main publications in international journals

51. Wang B, Ma YL, Zhang ZB and Wang QC (2011) Cryopreservation of china's potato shoot tips by vitrification, encapsulation-vitrification and droplet-vitrification. First International Symposium on Horticultural crops in China. Yangling, Shaanxi, PR. China.

50. Yin ZF, Chen L, Zhao B, Zhu YQ and Wang QC (2011) In vitro shoot regeneration and cryopreservation of Lilium spp. by vitrification. First International Symposium on Horticultural crops in China. Yangling, Shaanxi, PR. China.

49. Feng CH, Cui ZH, Li BQ, Ma YL and Wang QC. 2011. Cryopreservation of in vitro-grown shoot tips of apple (Malus) by encapsulation-dehydration. First International Symposium on Horticultural crops in China. Yangling, Shaanxi, PR. China.

48. Zhang ZB, Wang B, Ma YL and Wang QC. 2011. Novel and potential application of cryopreservation to transgenic formation. Biotechnology Advances (submitted).

47. Yin ZF, Chen L, Zhao M and Wang QC. 2011. Cryopreservation of embryogenic cell suspensions by encapsulation-dehydration and encapsulation-vitrification. Humana Press (in press).

46. Wang B, Ma YL, Zhang ZB, Wu ZM, Wu YF, Wang QC, Li MF (2011) Potato viruses in China. Crop Protection. doi:10.1016/j.cropro.2011.04.001

45. Feng CH, Yin ZF, Zhang ZB, Ma YL, Wang B and Wang QC. 2011. Plant pathogen elimination by cryotherapy of shoot tips. Springer, Netherland (in press).

44. Feng CH, Yin ZF, Ma YL, Zhang ZB, Chen L, Li BQ, Huang YS, Wang B, and Wang QC. 2011. Cryopreservation of sweetpotato and its pathogen elimination by cryotherapy. Biotechnology Advances, 29: 84-93.

43. Feng CH, Ma YL, Zhang ZB, Wang B, Yin ZF, Chen L, Li BQ, Wang QC. 2010. Virus elimination of fruit trees by cryotherapy of shoot tips. Journal of Fruit Science. 27(suppl.): 17-21.

42. Yin ZF, Feng CH, Wang B and Wang QC. 2010. Cryotherapy of shoot tips: a newly emerging technique for efficient elimination of plant pathogens. Acta Horti. (in press).

41. Wang QM, Zhang LM, Wang B, Yin ZF, Feng CH and Wang QC. 2010. Sweetpotato viruses in China. Crop Protection. 29:110-114.

40. Nukari A，Uosukainen M, Rokka VM, Antonius K, Wang QC, Valkonen JPT (2009) Cryopreservation techniques and their application in vegetatively propagated crop plants in Finland. Agricultural and Food Science, 18: 117-128.

39. Wang QC, Panis B, Engelmann F, Lambardi M and Valkonen JPT (2009) Cryotherapy of shoot tips: a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation. Annals of Applied Biology. 154: 351-363

38. Wang QC and Valkonen JPT. 2009. Cryotherapy of shoot tips: novel pathogen eradication method. Trends in Plant Science, 14:119-122.

37. Wang QC and Valkonen JPT. 2009. Improved recovery of cryo-treated shoot tips following theromotherapy of in vitro stock shoots of raspberry (Rubus idaeus L.). CryoLetters, 30(3): 171-182.

36. Zhang LM, Wang QM, Liu QC and Wang QC. 2009. Sweetpotato in China. In: Biology and Biotechnology of Sweetpotato. Eds: Loebenstain G. and Thottappilly G, Springer Netherland, pp. 325-358.

35. Wang B, Yin ZF, Feng CH, Shi X, Li YP and Wang QC. 2009. Cryopreservation of potato shoot tips. In: Benkeblia N, Tennant P (Eds) Potato I. Fruit, Vegetable and Cereal Science and Biotechnology 2 (Special Issue 1), pp. 46-53, Global Science Book, London.

34. Wang QC and Valkonen JPT. 2008. Elimination of two synergistically interacting viruses from sweetpotato by shoot tip culture and cryotherapy of shoot tips. Journal of Virological Methods. 154, 135-145

33. Wang QC, Laamanen J, Nukari A, Uosukainen M, Kesulahti J and Valkonen JPT. 2008. Sailytettavan aineiston tautipuhdistus kryoterapialla. Plant Biotechnology in Finland. 4: 1-5

32. Nukari A, Wang QC, Uosukainen M, Laamanen J, Rokka V.-T, Rantala S and Valkonen JPT 2008. Evaluation of encapsulation and droplet vitrification methods in gene preservation work. Cryopreservation of crop species in Europe, CRYOPLANET - COST Action 871, February 2008, Oulu, Finland, pp. 55-56

31. Wang QC and Valkonen JPT. 2008. Efficient elimination of sweetpotato little leaf phytoplasma by cryoptherapy of shoot tips (Ipomoea batatas L.). Plant Pathology, 57:338-347.

30. Wang QC, Cuellar WJ, Rajamaki M-L, Hirata Y, and Valkonen J JP. 2008. Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Molecular Plant Pathology, 9:237-250.

29. Wang QC and Valkonen JPT. 2007. Elimination of viruses and phytoplasma by cryotherapy of in vitro-grown shoot tips of sweet potato and raspberry. An Invited Lecturer, European Cost Action Meeting. 11-12th of May, 2007. Florence, Italy.

28. Wang QC and Valkonen JPT. 2007. Elimination of viruses and phytoplasma by cryotherapy of in vitro-grown shoot tips: Analysis of all cases. Advances in Horticultural Science, 21: 265-269.

27. Wang QC and Valkonen JPT. 2007 Cryopreservation of in vitro-grown shoot tips of raspberry. In: Plant Cryopreservation of plants：A Practical Guide，R. Babara (ed). Springer-Valerg. pp. 333-365.

26. Wang QC. 2007 Cryopreservation of in vitro-grown shoot tips of grapevine. In: Plant Cryopreservation of plants：A Practical Guide, R. Babara (ed). Springer-Valerg. pp. 333-365.

25. Wang QC and Perl A. 2006. Cryopreservation in Floricultural Crops. In: Jaimie T. da Silva (ed), Floricultural, Ornamental and Plant Biotechnology: Advances and Topics. Global Science Books, London. Chapter 58. pp. 523-539.

24. Wang QC, Lui Y, Xie LR and You MS. 2006 Efficient elimination of Potato leafroll virus (PLV) and Potato virus Y (PVY) by cryopreservation of shoot tips. Potato Research. 49:119-129.

23. Wang QC and Perl A. 2005. Cryopreservation of embryogenic cell suspensions by encapsulation-vitrification. In: Víctor M. Loyola-Vargas and Felipe Vázquez-Flota, Plant Cell Culture Protocols, Methods in Molecular, Humana Press, USA, Chapter 17, pp.77-86.

22. Nukari A, Uosukainen M and Wang QC. 2005. Cryopreservation of quality plant production. In: COST843 and COST851 Joint Metting. June 28-July 2, 2005. Stará Lessná, Slovakia, pp 171-172.

21. Wang QC, Laamanen J, Uosukainen M and Valkonen JPT (2005) Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation-vitrification and encapsulation-dehydration. Plant Cell Rep. 24: 280-288.

20. Wang QC, Li P, Hanania U, Sahar N, Mawassi M, Gafny R, Sela I, Tanne E and Perl A. 2005. Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions. Plant Science, 168:565-571.

19. Wang QC, Mawassi M, Sahar N, Li P, Violeta C.-T, Gafny R, Sela I, Tanne E, Perl A (2004) Cryopreservation of grapevine (Vitis spp.) embryogenic cell suspensions by encapsulation-vitrification. Plant Cell, Tissue and Organ Culture, 77: 267-275.

18. Wang QC, Li P, Batuman O, Gafny R and Mawassi M. 2003. Effect of benzyladenine on recovery of cryopreserved shoot tips of grapevine and citrus cultured in vitro. Cryoletters, 24: 293-302.

17. Wang QC, Mawassi M, Li P, Gafny R, Sela I and Tanne E. 2003. Elimination of grapevine virus A (GVA) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L. Plant Science, 165:321-327.

16. Wang QC, Gafny R, Sahar N, Sela I, Mawassi M, Tanne E and Perl A. 2002. Cryopreservation of grapevine (Vitis vinifera L.) embryogenic cell suspensions by encapsulation-dehydration and subsequent plant regeneration. Plant Science, 162: 551-558.

15. Wang QC, Batuman ?, Li P, Bar-Joseph M and Gafny R. 2002. A simple and efficient cryopreservation of in vitro-grown shoot tips of `Troyer' citrange [Poncirus trifoliata (L.) Raf. × Citrus sinensis (L.) Osbeck.] by encapsulation-vitrification. Euphytica, 128:135-142.

14. Wang QC, Batuman ?, Li P, Bar-Joseph M and Gafny R. 2002 Cryopreservation of in vitro-grown shoot tips of 'Troyer' citrange [poncirus trifoliata (L.) Raf. X Citrus sinensis (L.) Osbeck.] by encapsulation-dehydration. Plant Cell Reports. 20:901-906.

13. Wang QC, Tanne E, Arav A and Gafny R. 2000. Cryopreservation of in vitro-grown shoot tips of grapevine by encapsulation-dehydration. Plant Cell, Tissue and Organ Culture, 63:41-46.

12. Deng GT, Li DF, Li YT, Zhang YS, Wang QC, Xiu LH and Zhang FL. 1995. Distribution and use of Malus in Hengduan Mountain region, southwest China. Plant Genetic Resources Newsletter, 102: 29-32.

11. Knapp E, da Camara Machado A, Puhringer H, Wang QC, Hanzer V, Weiss H, Weiss B, Katinger H and Laimer da Camara Machado M. 1995. Localization of fruit tree viruses by immuno-tissue printing in infected shoots of Malus and Prunus sp. Journal of Virological Methods. 55(2):157-173.

10. Knapp E, Hanzer V, Weiss H, da Camara Machado A, Wang QC, Weiss B, Katinger H and Laimer da Camara Machado M. 1995. Distribution of ACLSV in apple shoots cultivated in vitro. XVI ISHS Symposium on Fruit Tree Viruses. Rome, 1994, Acta Horticulturae 386: 187-194.

9. Knapp E, Hanzer V, Weiss H, da Camara Machado A, Wang QC, Weiss B, Katinger H and Laimer da Camara Machado M. 1995. New Aspects of virus elimination in fruit trees. XVI ISHS Symposium on Fruit Tree Viruses. Rome, 1994, Acta Horticulturae 386: 409-418

8. Wang QC and Tang HR. 1994. Strawberry culture in China. Chronic Horticulturae. 34: 4-7.

7. Wang QC, Tang HR, Quan Y and Zhou GR. 1994. Phenol induced browning and establishment of shoot-tip explants of 'Fuji' apple and 'Jinhua' pear cultured in vitro. Journal of Horticultural Science, 69: 833-839.

6. Wang QC. 1992. The effect of light, darkness and temperature on micropropagation of the pear rootstock BP10030. Journal of Horticultural Science, 67: 869-876.

5. Wang QC. 1991. Factors affecting rooting of microcuttings of the pear rootstock BP10030. Scientia Horticulturae, 45: 209-213.

4. Wang QC. 1991. Shoot multiplication of pear in double-phase medium culture, Acta Horticulturae, 289: 349-350.

3. Wang QC and Svensson M. 1988. Effect of double-phase medium on shoot multiplication of pear (Pyrus communis L.), Abstract Book of Second Nordic Symposium on Plant Tissue Culture, pp56-59.

2. Wang QC. 1988. Micropropagation of pear (Pyrus communis L.): factors affecting establishment of in vitro culture. Annal Report of Horticultural Crop Breeding, Swedish University of Agricultural Sciences, 6:93-99, Sweden

1. Wang QC and Andersen AS. 1998. Propagation of Hibiscus rosa-sinensis: relations between stock plant cultivar, age, environment and growth regulator treatment. Acta Horticulturae, 251: 289-309.