華東理工大學(xué)

華東理工大學(xué)生物工程學(xué)院生物工程研究生導(dǎo)師高蓓介紹如下：

生物化工專業(yè)：碩士生導(dǎo)師

生物工程專業(yè)：碩士生導(dǎo)師

生物工程領(lǐng)域：工程碩士生導(dǎo)師

高蓓，女，1982年10月生，博士，華東理工大學(xué)生物工程學(xué)院碩士生導(dǎo)師。長(zhǎng)期致力于合成生物學(xué)、分子生物學(xué)、生物催化與生物轉(zhuǎn)化等領(lǐng)域相關(guān)研究工作。作為負(fù)責(zé)人先后主持國(guó)家科技部“863”計(jì)劃、國(guó)家自然科學(xué)基金、上海市自然科學(xué)基金、中央高�；�本科研業(yè)務(wù)費(fèi)探索基金等多項(xiàng)國(guó)家及省部級(jí)課題，并與恒瑞醫(yī)藥股份有限公司、漢康豆類食品有限公司等多家企業(yè)進(jìn)行合作。在“Scientific Reports”、“Catalysis Science and Technology”、“FEBS Journal”、“Journal of Biotechnology”等本領(lǐng)域重點(diǎn)期刊上發(fā)表論文多篇，授權(quán)專利多項(xiàng)。
 
教育經(jīng)歷：

2005-2010 華東理工大學(xué)博士

2001-2005 燕山大學(xué)學(xué)士

主要研究方向：

合成生物學(xué)：基于合成生物學(xué)和代謝工程的原理和方法，致力于微生物細(xì)胞工廠的開(kāi)發(fā)與應(yīng)用；

酶工程：基于計(jì)算生物學(xué)和結(jié)構(gòu)生物學(xué)的酶資源開(kāi)發(fā)與理性改造；基于生物化學(xué)和生物催化與轉(zhuǎn)化的工業(yè)化酶的創(chuàng)制及應(yīng)用技術(shù)研究。

代表性論文：

1.Min Jiang, Lujia Zhang, Fengqing Wang, Jie Zhang, Guosong Liu, Bei Gao, Dongzhi Wei. Novel application of magnetic protein: convenient one-step purification and immobilization of proteins. Scientific Reports. 2017, DOI:10.1038/s41598-017-13648-x.

2.BeiGao, Shuiqin Jiang, Liuzhu Wang, Lujia Zhang, Dongzhi Wei. Energy and conformation determine the enantioselectivity of enzyme. Biochemical Engineering Journal. 2018, 106-112.

3.Lei He, Youzhi Mao, Lujia Zhang, Hualei Wang, SitiAisyah Alias, Bei Gao, Dongzhi Wei. Functional expression of a novel α-amylase from Antarctic psychrotolerant fungus forbaking industry and its magneticimmobilization. BMC Biotechnology. 2017, 17:22.

4.Shuiqin Jiang, Lujia Zhang, Zhiqiang Yao, Bei Gao, Hualei Wang, Dongzhi Wei. Switching a nitrilase from Syechocystis sp. PCC6803 to a nitrile hydratase by rationally regulating reaction pathways<!--[if !supportNestedAnchors]-->. Catalysis Science and Technology. 2017, 7(5):1122-1128.

5.Shuiqin Jiang, Lujia Zhang, Dongbin Cui, Zhiqiang Yao, Bei Gao, Jinping Lin, Dongzhi Wei. The important role of halogenbond in substrate selectivity ofenzymatic catalysis. Scientific Reports. 2016, 6: 34750.

6.Zhiqiang Yao, Lujia Zhang, Bei Gao, Dongbing Cui, Fengqing Wang, Xiao He, Dongzhi Wei. A semiautomated structure-based method to predict substrates of enzymes via molecular docking: a case study with Candida antarctica lipase B. Journal of Chemical Information and Modeling. 2016, 56: 1979-1994.

7.Wenyuan Gao, Kai Wu, Lifeng Chen, Haiyang Fan, Zhiqiang Zhao, Bei Gao, Hualei Wang, Dongzhi Wei. A novel esterase from a marine mud metagenomics library for biocatalytic synthesis of short chain flavor esters. Microbial Cell Factories. 2016, 15: 41-52

8.BeiGao, Youzhi Mao, Lujia Zhang, Lei He, Dongzhi Wei. A novel saccharifying α-amylase of Antarctic psychrotolerant fungi Geomycespannorum: Gene cloning, functional expression, and characterization．Starch/Stärke. 2015, 67(1): 1-9.

9.Youzhi Mao, Yanchen Yin, Lujia Zhang, SitiAisyah Alias, Bei Gao , Dongzhi Wei. Development of a novel Aspergillus uracil deficient expression systemand its application in expressing a cold-adapted α-amylase gene fromAntarctic fungi Geomycespannorum. Process Biochemistry. 2015, 50: 1581-1590

10.Yanchen Yin, Youzhi Mao, Xiaolie Yin, BeiGao, Dongzhi Wei. Construction of a shuttle vector for heterologous expression of a novel fungal α-amylase gene in Aspergillus oryzae．Journal of Microbiology and Biotechnology. 2015,25(7):988-998.

11.Dongbing Cui,Lujia Zhang, Shuiqin Jiang,Zhiqiang Yao,Bei Gao,Jinping Lin,Y. Adam Yuan,Dongzhi Wei. A computational strategy for altering an enzyme in its cofactor preference to NAD(H) and/or NADP(H). FEBS Journal. 2015, 282(12): 2339-2351.

12.Zhenggui He, Lujia Zhang, Youzhi Mao, JingchaoGu, Qi Pan, Sixing Zhou, Bei Gao,Dongzhi Wei. Cloning of a novel thermostable glucoamylasefrom thermophilic fungus Rhizomucorpusillus andhigh-level co-expression withα-amylase in Pichiapastoris. BMC Biotechnology. 2014, 14:114

13.Zhenggui He, Yanchen Yin, Youzhi Mao, Sixing Zhou, Qi Pan, Dongzhi Wei, Bei Gao. Cloning and high-level expression of thermostable α-amylase gene from Rhizomucorpusillus. Journal of Southern Agriculture. 2014, 45(2): 165-172

14.Lujia Zhang,Xiaomang Tang,Dongbing Cui, Zhiqiang Yao,Bei Gao,Shuiqin Jiang,Bo Yin,Y. Adam Yuan,Dongzhi Wei. A method to rationally increase protein stability based on the charge-charge interaction, with application to lipase LipK107. Protein Science. 2014, 23:110-116.

15.Lujia Zhang, Bei Gao, Zuanning Yuan, Xiao He, Y. Adam Yuan, John Z.H. Zhang,Dongzhi Wei. Structure,mechanism, and enantioselectivity shifting of lipase LipK107 with a simple way.BiochimicaetBiophysicaActa (BBA) -Proteins and Proteomics. 2014, 1844:1183-1192.

16.Xiangping Wu, Pengyong You, Erzheng Su, Jingjing Xu, BeiGao,Dongzhi Wei. In vivo functional expression of a screenedP. aeruginosachaperone-dependent lipase in E. coli.BMC Biotechnology, 2012, 12: 58-64.

17.Bei Gao, Tao Xu, Jinping Lin, Lujia Zhang, Zhengbing Jiang, Dongzhi Wei. Improving the catalytic activity of lipase LipK107 from Proteus sp. by site-directed mutagenesis in the lid domain based on computer simulation. Journal of Molecular Catalysis B: Enzymatic, 2011, 68: 286–291.

18.Huiting Song, Lichao Zhou, Lujia Zhang, Bei Gao,Dongzhi Wei, Yaling Shen, Rui Wang, Catherine Madzak, Zhengbing Jiang, Construction of a whole-cell catalyst displaying a fungal lipase for effectivetreatment of oily wastewaters. Journal of Molecular Catalysis B: Enzymatic.2011, 71:166-170.

19.Tao Xu, Bei Gao, Lujia Zhang, Jingpin Lin, Xuedong Wang, Dongzhi Wei. Template-based modeling of a psychrophilic lipase: conformational changes, novel structural features and its application in predicting the enantioselectivity of lipase catalyzed transesterification of secondary alcohols. BiochimicaetBiophysicaActa(BBA) - Proteins & Proteomics, 2010, 1804: 2183-2190.

20.BeiGao, Erzheng Su, Jinping Lin, Dongzhi Wei. Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp. for biodiesel production.Journal of Biotechnology, 2009, 139(2):169-175.

21.Zhengbing Jiang, Bei Gao,Ren Ren, Dongzhi Wei．Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparision with the LipB52 displayed on Saccharomyces cereisiae cell surface．BMC Biotechnology, 2008, 8:4: 1-7.

專利：

1、魏東芝，高蓓，何正貴。一種共表達(dá)葡萄糖淀粉酶和α-淀粉酶的重組畢赤酵母菌株及其構(gòu)建方法，以及混合酶制劑。授權(quán)專利號(hào)：ZL201310033964.1

2、高蓓，魏東芝，何正貴。一種耐熱葡萄糖淀粉酶及其編碼基因與應(yīng)用。授權(quán)專利號(hào)：ZL201310016408.3

3、魏東芝，高蓓，馬昱澍，蘇二正。脂肪酶基因以及工程菌和脂肪酶菌株。授權(quán)專利號(hào)：ZL200810033414.9

4、魏東芝，高蓓，毛佑志。一種用于在米曲霉細(xì)胞分泌表達(dá)外源蛋白的表達(dá)設(shè)備及其米曲霉基因工程菌。授權(quán)專利號(hào)：ZL201410260946.1

5、魏東芝，高蓓，賀磊，張魯嘉。一種α-淀粉酶及其編碼基因與應(yīng)用。專利公開(kāi)號(hào)：201610052870.2

6、高蓓，魏東芝，賀磊，張魯嘉。一種天冬氨酸類蛋白酶及其編碼基因與應(yīng)用。專利公開(kāi)號(hào)：201610052686.8

7、高蓓，魏東芝，張魯嘉，王風(fēng)清，江敏，劉國(guó)松。一種利用磁感應(yīng)蛋白作為純化標(biāo)簽實(shí)現(xiàn)外源蛋白一步純化與固定化的方法，應(yīng)用及通用載體。專利公開(kāi)號(hào)：201710444356.8

承擔(dān)課題：

1、2018-2021 國(guó)家自然科學(xué)基金面上項(xiàng)目 基于底物選擇性機(jī)制解析的脂肪酶理性設(shè)計(jì)及ω-3多不飽和脂肪酸甘油酯的定向合成（31772007）直接經(jīng)費(fèi)60萬(wàn)元，課題負(fù)責(zé)人。

2、2016-2019 上海市自然科學(xué)基金基于結(jié)構(gòu)認(rèn)知和理論計(jì)算的酶分子理性設(shè)計(jì)策略研究（16ZR1449500）20萬(wàn)元，課題負(fù)責(zé)人。

3、2013-2017 國(guó)家高技術(shù)發(fā)展（863）計(jì)劃 啤酒用新酶創(chuàng)制與低碳制造關(guān)鍵技術(shù)研究 （2013AA102109）80萬(wàn)元，子課題負(fù)責(zé)人。

4、2015-2017 浙江省近岸水域生物資源開(kāi)發(fā)與保護(hù)重點(diǎn)實(shí)驗(yàn)室開(kāi)放基金項(xiàng)目 可口革囊星蟲(chóng)降血壓肽的分子機(jī)制（J2015001）3萬(wàn)元，課題負(fù)責(zé)人。

5、2013-2015 國(guó)家自然科學(xué)基金青年項(xiàng)目基于結(jié)構(gòu)生物學(xué)及分子模擬方法的脂肪酶理性設(shè)計(jì)與改造（31201296）23萬(wàn)元，課題負(fù)責(zé)人。

6、2015-2016 中央直屬高�；�本科研業(yè)務(wù)費(fèi)專項(xiàng)基金探索研究基金 基于量化計(jì)算的酶催化體系反應(yīng)過(guò)程分析新方法、新策略研究（WF1414038）16萬(wàn)元，課題負(fù)責(zé)人。

7、2012-2013 中央直屬高校基本科研業(yè)務(wù)費(fèi)專項(xiàng)基金探索研究基金 脂肪酶的理性設(shè)計(jì)與改造（WF1114022）12萬(wàn)元，課題負(fù)責(zé)人。

8、2012-2014江蘇恒瑞醫(yī)藥股份有限公司橫向課題，胰蛋白酶、羧肽酶B酶制劑的開(kāi)發(fā)50萬(wàn)元，課題負(fù)責(zé)人。

9、2016-2019 國(guó)家自然科學(xué)基金面上項(xiàng)目基于Pgrac啟動(dòng)子突變文庫(kù)篩選及元件組裝的芽孢桿菌精細(xì)調(diào)控表達(dá)系統(tǒng)的研究（31570795）77.5萬(wàn)元，主要參與人。

10、2012-2015 國(guó)家高技術(shù)發(fā)展（863）計(jì)劃酶分子規(guī)模化改造計(jì)算設(shè)計(jì)關(guān)鍵技術(shù)研究及應(yīng)用 （2012AA020403）主要參與人。

11、2009-2012 國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃（973）計(jì)劃 新一代生物催化和生物轉(zhuǎn)化的科學(xué)基礎(chǔ) （2009CB724703）主要參與人。

12、2012-2016 華東理工大學(xué)交叉學(xué)科與重大項(xiàng)目培育基金葡萄糖衍生物生物合成系統(tǒng)的元件解析與系統(tǒng)創(chuàng)建，主要參與人。

聯(lián)系方式：

電話：021-64251803，18019078882

傳真：021-64250068

微信：18019078882

郵箱：gaobei@ecust.edu.cn

通訊地址：生物工程學(xué)院 生物反應(yīng)器國(guó)家重點(diǎn)實(shí)驗(yàn)室18樓707室

上海市徐匯區(qū) 梅隴路130號(hào) 華東理工大學(xué)

郵編：200237